The study of crustal and lithospheric thicknesses provides valuable information about the Earth’s dynamics, allowing to identify stress patterns, isostatic compensation degrees and to generate crustal evolution models. Lithospheric thickness in South America is poorly understood, being the Andean region the most studied. The aim of this work is to evaluate the lithospheric structure of the Pantanal basin analyzing S-wave velocity models obtained from the fundamental and higher modes of surface wave from regional earthquakes recorded at the Brazilian Seismic Network Stations (BRASIS). Surface wave group velocity curves were obtained by a multiple filter technique and the classical two-station method was applied to get the fundamental and higher mode phase velocity curves. Inversions of these curves were carried out to obtain the velocity profile for the lithospheric region.  This new models reach depths up to 150 km enhancing the lithospheric structure knowledge. This work represents the first analysis of phase velocity using higher modes for the Pantanal basin.